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Adhesion of hFOB 1.19 cells on DLP-printed 3Y and 5Y zirconia surfaces with and without meso-scale modifications. Cell adhesion after 4 h ( A ) and 24 h ( B ) is shown relative to tissue culture plastic (TCP), which was set to 100%. Meso-scale modified surfaces (Mod-80, Mod-120, Mod-160) generally exhibited significantly higher cell adhesion compared with unmodified zirconia for both 3Y and 5Y materials, with differences becoming more pronounced after 24 h. A reduction in cell adhesion from 4 h to 24 h was observed mainly on unmodified and Mod-80 and Mod-120 3Y surfaces, whereas Mod-160 3Y and 5Y surfaces sustained cell adhesion over time. Significant differences were also detected between specific meso-scale feature sizes, indicating an influence of meso-scale structural design on the maintenance of cell adhesion. Significant differences between groups are indicated by square brackets; for clarity, asterisks denoting statistical significance were omitted. Kruskal–Wallis One-Way Analysis of Variance on Ranks, Bonferroni post hoc test, n = 9.
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Adhesion of hFOB 1.19 cells on DLP-printed 3Y and 5Y zirconia surfaces with and without meso-scale modifications. Cell adhesion after 4 h ( A ) and 24 h ( B ) is shown relative to tissue culture plastic (TCP), which was set to 100%. Meso-scale modified surfaces (Mod-80, Mod-120, Mod-160) generally exhibited significantly higher cell adhesion compared with unmodified zirconia for both 3Y and 5Y materials, with differences becoming more pronounced after 24 h. A reduction in cell adhesion from 4 h to 24 h was observed mainly on unmodified and Mod-80 and Mod-120 3Y surfaces, whereas Mod-160 3Y and 5Y surfaces sustained cell adhesion over time. Significant differences were also detected between specific meso-scale feature sizes, indicating an influence of meso-scale structural design on the maintenance of cell adhesion. Significant differences between groups are indicated by square brackets; for clarity, asterisks denoting statistical significance were omitted. Kruskal–Wallis One-Way Analysis of Variance on Ranks, Bonferroni post hoc test, n = 9.
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Adhesion of hFOB 1.19 cells on DLP-printed 3Y and 5Y zirconia surfaces with and without meso-scale modifications. Cell adhesion after 4 h ( A ) and 24 h ( B ) is shown relative to tissue culture plastic (TCP), which was set to 100%. Meso-scale modified surfaces (Mod-80, Mod-120, Mod-160) generally exhibited significantly higher cell adhesion compared with unmodified zirconia for both 3Y and 5Y materials, with differences becoming more pronounced after 24 h. A reduction in cell adhesion from 4 h to 24 h was observed mainly on unmodified and Mod-80 and Mod-120 3Y surfaces, whereas Mod-160 3Y and 5Y surfaces sustained cell adhesion over time. Significant differences were also detected between specific meso-scale feature sizes, indicating an influence of meso-scale structural design on the maintenance of cell adhesion. Significant differences between groups are indicated by square brackets; for clarity, asterisks denoting statistical significance were omitted. Kruskal–Wallis One-Way Analysis of Variance on Ranks, Bonferroni post hoc test, n = 9.
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normal osteoblast cell line - by Bioz Stars, 2026-10
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Adhesion of hFOB 1.19 cells on DLP-printed 3Y and 5Y zirconia surfaces with and without meso-scale modifications. Cell adhesion after 4 h ( A ) and 24 h ( B ) is shown relative to tissue culture plastic (TCP), which was set to 100%. Meso-scale modified surfaces (Mod-80, Mod-120, Mod-160) generally exhibited significantly higher cell adhesion compared with unmodified zirconia for both 3Y and 5Y materials, with differences becoming more pronounced after 24 h. A reduction in cell adhesion from 4 h to 24 h was observed mainly on unmodified and Mod-80 and Mod-120 3Y surfaces, whereas Mod-160 3Y and 5Y surfaces sustained cell adhesion over time. Significant differences were also detected between specific meso-scale feature sizes, indicating an influence of meso-scale structural design on the maintenance of cell adhesion. Significant differences between groups are indicated by square brackets; for clarity, asterisks denoting statistical significance were omitted. Kruskal–Wallis One-Way Analysis of Variance on Ranks, Bonferroni post hoc test, n = 9.

Journal: Bioengineering

Article Title: Meso-Scale Modifications in Additively Manufactured Zirconia: Topographical Design and Its Influence on Cell–Material Interactions

doi: 10.3390/bioengineering13050498

Figure Lengend Snippet: Adhesion of hFOB 1.19 cells on DLP-printed 3Y and 5Y zirconia surfaces with and without meso-scale modifications. Cell adhesion after 4 h ( A ) and 24 h ( B ) is shown relative to tissue culture plastic (TCP), which was set to 100%. Meso-scale modified surfaces (Mod-80, Mod-120, Mod-160) generally exhibited significantly higher cell adhesion compared with unmodified zirconia for both 3Y and 5Y materials, with differences becoming more pronounced after 24 h. A reduction in cell adhesion from 4 h to 24 h was observed mainly on unmodified and Mod-80 and Mod-120 3Y surfaces, whereas Mod-160 3Y and 5Y surfaces sustained cell adhesion over time. Significant differences were also detected between specific meso-scale feature sizes, indicating an influence of meso-scale structural design on the maintenance of cell adhesion. Significant differences between groups are indicated by square brackets; for clarity, asterisks denoting statistical significance were omitted. Kruskal–Wallis One-Way Analysis of Variance on Ranks, Bonferroni post hoc test, n = 9.

Article Snippet: The experimental procedures intend the analysis of cell–material interactions utilized the human fetal osteoblast cell line hFOB 1.19 (CRL-11372; American Type Culture Collection, ATCC ® , Manassas, VA, USA).

Techniques: Modification

Proliferation of hFOB 1.19 cells on DLP-printed 3Y ( A ) and 5Y ( B ) zirconia surfaces with and without meso-scale structural modifications. Cell numbers were determined after 4 h (day 0) and daily for a total of 4 days. Growth curves are shown for unmodified and meso-scale structurally modified surfaces (Mod-80, Mod-120, Mod-160) and compared with cells cultured on standard tissue culture plastic (control). No significant differences in proliferation rates, assessed by comparison of growth curve slopes, were observed between surface modifications, zirconia materials, or the control, indicating comparable cell growth on all tested surfaces.

Journal: Bioengineering

Article Title: Meso-Scale Modifications in Additively Manufactured Zirconia: Topographical Design and Its Influence on Cell–Material Interactions

doi: 10.3390/bioengineering13050498

Figure Lengend Snippet: Proliferation of hFOB 1.19 cells on DLP-printed 3Y ( A ) and 5Y ( B ) zirconia surfaces with and without meso-scale structural modifications. Cell numbers were determined after 4 h (day 0) and daily for a total of 4 days. Growth curves are shown for unmodified and meso-scale structurally modified surfaces (Mod-80, Mod-120, Mod-160) and compared with cells cultured on standard tissue culture plastic (control). No significant differences in proliferation rates, assessed by comparison of growth curve slopes, were observed between surface modifications, zirconia materials, or the control, indicating comparable cell growth on all tested surfaces.

Article Snippet: The experimental procedures intend the analysis of cell–material interactions utilized the human fetal osteoblast cell line hFOB 1.19 (CRL-11372; American Type Culture Collection, ATCC ® , Manassas, VA, USA).

Techniques: Modification, Cell Culture, Control, Comparison

Osteogenic differentiation-associated gene expression of hFOB 1.19 cells cultured on DLP-printed 3Y and 5Y zirconia surfaces with and without meso-scale structural modifications. Cells were analyzed after 1, 2, and 7 days. Relative mRNA expression levels of early (RUNX2, ( A )), intermediate (ALPL, ( B ); COL1A1, ( C )), and late (BGLAP, ( D )) osteogenic markers were quantified by qPCR. Meso-scale structural modifications (Mod-80, Mod-120 and Mod-160) induced material- and feature size-dependent differences in gene expression, with pronounced early and sustained induction of BGLAP on modified 3Y zirconia, particularly on Mod-160, whereas 5Y zirconia showed more moderate or absent differentiation-related responses. Significant differences between groups are indicated by square brackets; asterisks were omitted for clarity. Kruskal–Wallis One-Way Analysis of Variance on Ranks, Bonferroni post hoc test, n = 3.

Journal: Bioengineering

Article Title: Meso-Scale Modifications in Additively Manufactured Zirconia: Topographical Design and Its Influence on Cell–Material Interactions

doi: 10.3390/bioengineering13050498

Figure Lengend Snippet: Osteogenic differentiation-associated gene expression of hFOB 1.19 cells cultured on DLP-printed 3Y and 5Y zirconia surfaces with and without meso-scale structural modifications. Cells were analyzed after 1, 2, and 7 days. Relative mRNA expression levels of early (RUNX2, ( A )), intermediate (ALPL, ( B ); COL1A1, ( C )), and late (BGLAP, ( D )) osteogenic markers were quantified by qPCR. Meso-scale structural modifications (Mod-80, Mod-120 and Mod-160) induced material- and feature size-dependent differences in gene expression, with pronounced early and sustained induction of BGLAP on modified 3Y zirconia, particularly on Mod-160, whereas 5Y zirconia showed more moderate or absent differentiation-related responses. Significant differences between groups are indicated by square brackets; asterisks were omitted for clarity. Kruskal–Wallis One-Way Analysis of Variance on Ranks, Bonferroni post hoc test, n = 3.

Article Snippet: The experimental procedures intend the analysis of cell–material interactions utilized the human fetal osteoblast cell line hFOB 1.19 (CRL-11372; American Type Culture Collection, ATCC ® , Manassas, VA, USA).

Techniques: Gene Expression, Cell Culture, Expressing, Modification